蛋白芯片详细

信号通路磷酸化抗体芯片 > Cancer/Apoptosis肿瘤凋亡磷酸化抗体芯片(PAC155)

Cancer/Apoptosis肿瘤凋亡磷酸化抗体芯片(PAC155)

     

        Cancer/Apoptosis磷酸化抗体芯片 (PAC155) 凋亡是最为著名的细胞程序性死亡过程，它调控着组织的平衡状态。凋亡蛋白与肿瘤的发生发展及药物开发的关系一直备受科学界关注。PAC155抗体芯片采用三维高分子膜专利技术，在抗体芯片片基上共价结合155种高特异抗体，针对肿瘤相关的著名凋亡蛋白的磷酸化位点进行广泛筛选。针对每一个特定蛋白磷酸化位点，设置一对抗体分别检测其磷酸化（Phospho）和非磷酸化（non-Phospho）状态。同时，该抗体芯片运用特有的荧光标记技术进行样本标记，实现了对肿瘤相关凋亡蛋白的高效精确检测。               

                                                                            

抗体芯片特点：

 

1. 芯片规格为76 x 25 x 1 mm；

2. 实现单一信号通路全面筛选；

3. 每种抗体设置6次技术重复；

4. 适用于组织、细胞等多类型样本；

5. 5 x106细胞、200 μg总蛋白量即可满足实验；

6. 每个检测位点设有磷酸化和非磷酸化配对抗体；

7. 可通用于人、小鼠、大鼠等多类型模式生物检测。

 

抗体芯片原理：

 

                

抗体芯片列表：

 

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抗体芯片文献：


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9.        Luo L L, Zhao L, Wang Y X, et al. Insulin-like growth factor binding protein-3 is a new predictor of radiosensitivity on esophageal squamous cell carcinoma. Sci Rep-UK, 2015, 5: 17336.  (中山大学附属肿瘤医院)

10.       Zhu Y P, Wan F N, Shen Y J, et al.  Reactive stroma component COL6A1 is upregulated in castration-resistant prostate cancer and promotes tumor growth. Oncotarget, 2015, 6(16): 14488.（复旦大学附属肿瘤医院）
11.      Wang T, Han S, Wu Z, et al.  XCR1 promotes cell growth and migration and is correlated with bone metastasis in non-small cell lung cancer. Biochem Bioph Res Co, 2015, 464(2): 635-641. （上海长征医院）

12.      Chen P, Huang H, Wu J, et al. Bone marrow stromal cells protect acute myeloid leukemia cells from anti‐CD44 therapy partly through regulating PI3K/Akt-p27(Kip1) axis. Mol Carcinogen, 2015, 54(12): 1678-85. (福建医科大学附属协和医院)

13.      Wan F, et al. Oxidized low-density lipoprotein is associated with advanced-stage prostate cancer. Tumor Biol, 2015: 1-10. (复旦大学附属肿瘤医院)

14.      Zhu R, et al. pH sensitive nano layered double hydroxides reduce the hematotoxicity and enhance the anticancer efficacy of etoposide on non-small cell lung cancer.Acta Biomater, 2016, 29: 320-32.  (同济大学)

15.      Li W, et al.  Withaferin A suppresses the up-regulation of acetyl-coA carboxylase 1 and skin tumor formation in a skin carcinogenesis mouse model. Mol Carcinog, 2015, 55(11): 1739-1746. (河北大学)

16.      Rao W, et al. OVA66 increases cell growth, invasion and survival via regulation of IGF-1R-MAPK signaling in human cancer cells. Carcinogenesis,2014, 35(7): 1573-1581.（上海交通大学医学院）

17.      Jia D, et al. Amplification of MPZL1/PZR promotes tumor cell migration through Src-mediated phosphorylation of cortactin in hepatocellular carcinoma. Cell Res, 2014, (24): 204-217. (上海仁济医院) 

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19.      Li F et al. Superoxide Mediates Direct Current Electric Field-Induced Directional Migration of Glioma Cells through the Activation of AKT and ERK. PLoS ONE. 2013, 8(4): e61195.（第三军医大学）

20.      Ranzato E et al. Epithelial mesenchymal transition traits in honey-driven keratinocyte wound healing: comparison among different honeys. Wound Repair Regen. 2012, 20(5): 778-785.

21.       Zhang YM et al. A novel angiogenesis inhibitor impairs lovo cell survival via targeting against human VEGFR and its signaling pathway of phosphorylation. Cell Death Dis, 2012, 3: e406.（西安交通大学）

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