蛋白芯片详细

信号通路磷酸化抗体芯片 > Cancer Signaling > 肿瘤信号通路磷酸化广谱筛选抗体芯片(CSP100)

肿瘤信号通路磷酸化广谱筛选抗体芯片(CSP100)

      肿瘤,在增殖、分化、凋亡、侵袭、炎症等方面具有复杂的生物学行为,是目前最难攻克的疾病之一。信号通路在肿瘤细胞自身生命活动,以及与基质细胞信号交往中起着关键作用,而每种胞行为的改变都存在多条信号通路的共同参与。层出不穷的抗癌药物耐受现象表明,单一信号通路的调节方式不能从根本上解决肿瘤治疗的问题。对肿瘤相关信号通路的整体把握和筛选,也因此成为肿瘤相关科学研究的起点。

                                           

       肿瘤信号通路磷酸化广谱筛选抗体芯片(CSP100,采用三维高分子膜专利技术,在片基上共价结合  269 种高特异抗体,分别检测93个信号蛋白的132个磷酸化位点。这些信号蛋白广泛参与多条与肿瘤相关信号通路的传导过程。芯片针对每一个特定蛋白磷酸化位点,设置一对抗体分别检测其磷酸化(Phospho)和非磷酸化(non-Phospho)状态,以提高磷酸化检测灵敏度和稳定性。一次芯片实验即可实现多条信号通路的同步筛选和关键调变位点的清晰定位,在肿瘤的发生、发展、转移、耐药、复发、微环境等机制研究发挥重要作用。同时,由于疾病信号通路的共性特点,CSP100也成为其它疾病研究中信号通路筛选的重要工具。

                                        

抗体芯片特点:        
       

  • 芯片规格为76 x 25 x 1 mm;

  • 实现12条信号通路全面筛选;

  • 每种抗体设置6次技术重复;

  • 适用于组织、细胞等多类型样本;

  • 5 x106细胞、200μg总蛋白量即可满足实验;

  • 每个检测位点设有磷酸化和非磷酸化配对抗体;

  • 可通用于人、小鼠、大鼠等多类型模式生物检测。

           

芯片检测列表:                

                                                       

  详细列表下载    
           


抗体芯片原理:
               

                                                       

                                        

                                                       

                                        

                                       

                   

抗体芯片文献:                  
     

      
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